LOCUS       40924_23384       10700 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB601 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Nakamura S, Mano S, Tanaka Y, Ohnishi M, Nakamori C, Araki M, Niwa 
            T, Nishimura M, Kaminaka H, Nakagawa T, Sato Y, Ishiguro S.
  TITLE     Gateway binary vectors with the bialaphos resistance gene, bar, as a
            selection marker for plant transformation
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (6), 1315-1319 (2010)
  PUBMED    20530878
REFERENCE   2  (bases 1 to 10700)
  AUTHORS   Tanaka Y, Nakamura S, Ishiguro S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 10700)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10700)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "6"; 
            pages: "1315-1319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..10700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1930..2054)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2086..2338
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2372..2388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2396..2412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2420..2450)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2465..2486)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(2522..2774)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2937..3485)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(3537..3716)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(4222..4246)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4767..5555
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5804..6392
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(6578..6718)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7062..7256)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7325..8395)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8827..9453)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcgcttag agctcgaatt tccccgatcg ttcaaacatt
     2101 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     2161 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     2221 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     2281 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     2341 gaattggttc cggaaccaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     2401 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     2461 cctaatgagt gagctaactc acattaggct gaattaggcg cgcctatttc tattaattcc
     2521 cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
     2581 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
     2641 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
     2701 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
     2761 atgtttgaac gatcggggaa attcgagctg gggacgtccc cagctcggta ccccctcctg
     2821 ggcacggtcg tacgcgatcc gccgcggttc cacctcacca ttgcgggtat gccagttgag
     2881 ttcgatgagt tcccgtacca gctccaactc gggcgatgcc acgcgcactc cgttcagatc
     2941 tcggtgacgg gcaggaccgg acggggcggt accggcaggc tgaagtccag ctgccagaaa
     3001 cccacgtcat gccagttccc gtgcttgaag ccggccgccc gcagcatgcc gcggggggca
     3061 tatccgagcg cctcgtgcat gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc
     3121 acgctcttga agccctgtgc ctccagggac ttcagcaggt gggtgtagag cgtggagccc
     3181 agtcccgtcc gctggtggcg gggggagacg tacacggtcg actcggccgt ccagtcgtag
     3241 gcgttgcgtg ccttccaggg gcccgcgtag gcgatgccgg cgacctcgcc gtccacctcg
     3301 gcgacgagcc agggatagcg ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt
     3361 tcctgcggct cggtacggaa gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg
     3421 cagaccgccg gcatgtccgc ctcggtggca cggcggatgt cggccgggcg tcgttctggg
     3481 ctcatatctt attgcccccc gggatcagat ctggattgag agtgaatatg agactctaat
     3541 tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct
     3601 agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct
     3661 tagctcatta aactccagaa acccgcggct gagtggctcc ttcaacgttg cggttctgtc
     3721 agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct
     3781 taattctccg ctcatgatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc
     3841 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
     3901 ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa
     3961 gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca
     4021 ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag
     4081 cagcccttgc gccctgagtg cttgcggcag cgtgaagctg atccgtcgag attaatagaa
     4141 ataaattcag cctaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt
     4201 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca
     4261 gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
     4321 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
     4381 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
     4441 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc
     4501 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     4561 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     4621 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     4681 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     4741 gtcgccctaa aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc
     4801 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     4861 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     4921 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     4981 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     5041 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     5101 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     5161 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     5221 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     5281 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     5341 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     5401 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     5461 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     5521 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca
     5581 agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt
     5641 gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag
     5701 ccctacacaa attgggagat atatcatgca tgaccaaaat cccttaacgt gagttttcgt
     5761 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5821 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5881 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5941 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6001 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6061 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6121 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6181 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6241 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6301 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6361 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6421 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6481 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6541 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     6601 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     6661 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     6721 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     6781 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     6841 accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg
     6901 cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag
     6961 cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag
     7021 gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg
     7081 ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt
     7141 ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt
     7201 acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa
     7261 agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac
     7321 attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg
     7381 ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca
     7441 gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga
     7501 tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga
     7561 gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg
     7621 ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact
     7681 ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg
     7741 ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg
     7801 tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc
     7861 ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct
     7921 tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt
     7981 cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg
     8041 gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg
     8101 ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa
     8161 aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg
     8221 gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg
     8281 cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt
     8341 catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga
     8401 ttcctcgggc ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac
     8461 gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg
     8521 ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt
     8581 tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc
     8641 cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga
     8701 cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg
     8761 cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt
     8821 aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc
     8881 gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac
     8941 gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac
     9001 ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct
     9061 tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat
     9121 gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac
     9181 agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc
     9241 cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc
     9301 ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc
     9361 ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc
     9421 gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt
     9481 actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat
     9541 caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc
     9601 agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg
     9661 ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa
     9721 aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct
     9781 cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc
     9841 tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat
     9901 gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc
     9961 gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg
    10021 ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg
    10081 caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg
    10141 gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta
    10201 ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc
    10261 gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct
    10321 cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct
    10381 ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg
    10441 cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa
    10501 gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca
    10561 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
    10621 ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa
    10681 atggacgaac ggataaacct
//
